Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses
Identifieur interne : 000E87 ( Main/Exploration ); précédent : 000E86; suivant : 000E88Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses
Auteurs : Sarah Watson [Australie] ; Sarah Mercier [Australie] ; Chris Bye [Australie] ; John Wilkinson [Australie] ; Anthony L. Cunningham [Australie] ; Andrew N. Harman [Australie]Source :
- Virology Journal [ 1743-422X ] ; 2007.
Abstract
The choice of an appropriate housekeeping gene for normalisation purposes has now become an essential requirement when designing QPCR experiments. This is of particular importance when using QPCR to measure viral and cellular gene transcription levels in the context of viral infections as viruses can significantly interfere with host cell pathways, the components of which traditional housekeeping genes often encode. In this study we have determined the reliability of 10 housekeeping genes in context of four heavily studied viral infections; human immunodeficiency virus type 1, herpes simplex virus type 1, cytomegalovirus and varicella zoster virus infections using a variety of cell types and virus strains. This provides researchers of these viruses with a shortlist of potential housekeeping genes to use as normalisers for QPCR experiments.
Url:
DOI: 10.1186/1743-422X-4-130
PubMed: 18053162
PubMed Central: 2216015
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000273
- to stream Pmc, to step Curation: 000273
- to stream Pmc, to step Checkpoint: 000674
- to stream Ncbi, to step Merge: 000106
- to stream Ncbi, to step Curation: 000106
- to stream Ncbi, to step Checkpoint: 000106
- to stream Main, to step Merge: 000E93
- to stream Main, to step Curation: 000E87
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses</title><author><name sortKey="Watson, Sarah" sort="Watson, Sarah" uniqKey="Watson S" first="Sarah" last="Watson">Sarah Watson</name><affiliation wicri:level="3"><nlm:aff id="I1">Centre for Virus Research, Westmead Millennium Institute, Sydney, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Centre for Virus Research, Westmead Millennium Institute, Sydney</wicri:regionArea><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Mercier, Sarah" sort="Mercier, Sarah" uniqKey="Mercier S" first="Sarah" last="Mercier">Sarah Mercier</name><affiliation wicri:level="3"><nlm:aff id="I1">Centre for Virus Research, Westmead Millennium Institute, Sydney, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Centre for Virus Research, Westmead Millennium Institute, Sydney</wicri:regionArea><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Bye, Chris" sort="Bye, Chris" uniqKey="Bye C" first="Chris" last="Bye">Chris Bye</name><affiliation wicri:level="4"><nlm:aff id="I2">The Howard Florey Institute, University of Melbourne, Melbourne, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>The Howard Florey Institute, University of Melbourne, Melbourne</wicri:regionArea><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName><orgName type="university">Université de Melbourne</orgName></affiliation></author><author><name sortKey="Wilkinson, John" sort="Wilkinson, John" uniqKey="Wilkinson J" first="John" last="Wilkinson">John Wilkinson</name><affiliation wicri:level="3"><nlm:aff id="I3">Biotron Limited, Centre for Immunology, St. Vincent's Hospital, Sydney, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Biotron Limited, Centre for Immunology, St. Vincent's Hospital, Sydney</wicri:regionArea><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Cunningham, Anthony L" sort="Cunningham, Anthony L" uniqKey="Cunningham A" first="Anthony L" last="Cunningham">Anthony L. Cunningham</name><affiliation wicri:level="3"><nlm:aff id="I1">Centre for Virus Research, Westmead Millennium Institute, Sydney, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Centre for Virus Research, Westmead Millennium Institute, Sydney</wicri:regionArea><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Harman, Andrew N" sort="Harman, Andrew N" uniqKey="Harman A" first="Andrew N" last="Harman">Andrew N. Harman</name><affiliation wicri:level="3"><nlm:aff id="I1">Centre for Virus Research, Westmead Millennium Institute, Sydney, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Centre for Virus Research, Westmead Millennium Institute, Sydney</wicri:regionArea><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">18053162</idno><idno type="pmc">2216015</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2216015</idno><idno type="RBID">PMC:2216015</idno><idno type="doi">10.1186/1743-422X-4-130</idno><date when="2007">2007</date><idno type="wicri:Area/Pmc/Corpus">000273</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000273</idno><idno type="wicri:Area/Pmc/Curation">000273</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000273</idno><idno type="wicri:Area/Pmc/Checkpoint">000674</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000674</idno><idno type="wicri:Area/Ncbi/Merge">000106</idno><idno type="wicri:Area/Ncbi/Curation">000106</idno><idno type="wicri:Area/Ncbi/Checkpoint">000106</idno><idno type="wicri:Area/Main/Merge">000E93</idno><idno type="wicri:Area/Main/Curation">000E87</idno><idno type="wicri:Area/Main/Exploration">000E87</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses</title><author><name sortKey="Watson, Sarah" sort="Watson, Sarah" uniqKey="Watson S" first="Sarah" last="Watson">Sarah Watson</name><affiliation wicri:level="3"><nlm:aff id="I1">Centre for Virus Research, Westmead Millennium Institute, Sydney, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Centre for Virus Research, Westmead Millennium Institute, Sydney</wicri:regionArea><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Mercier, Sarah" sort="Mercier, Sarah" uniqKey="Mercier S" first="Sarah" last="Mercier">Sarah Mercier</name><affiliation wicri:level="3"><nlm:aff id="I1">Centre for Virus Research, Westmead Millennium Institute, Sydney, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Centre for Virus Research, Westmead Millennium Institute, Sydney</wicri:regionArea><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Bye, Chris" sort="Bye, Chris" uniqKey="Bye C" first="Chris" last="Bye">Chris Bye</name><affiliation wicri:level="4"><nlm:aff id="I2">The Howard Florey Institute, University of Melbourne, Melbourne, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>The Howard Florey Institute, University of Melbourne, Melbourne</wicri:regionArea><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName><orgName type="university">Université de Melbourne</orgName></affiliation></author><author><name sortKey="Wilkinson, John" sort="Wilkinson, John" uniqKey="Wilkinson J" first="John" last="Wilkinson">John Wilkinson</name><affiliation wicri:level="3"><nlm:aff id="I3">Biotron Limited, Centre for Immunology, St. Vincent's Hospital, Sydney, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Biotron Limited, Centre for Immunology, St. Vincent's Hospital, Sydney</wicri:regionArea><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Cunningham, Anthony L" sort="Cunningham, Anthony L" uniqKey="Cunningham A" first="Anthony L" last="Cunningham">Anthony L. Cunningham</name><affiliation wicri:level="3"><nlm:aff id="I1">Centre for Virus Research, Westmead Millennium Institute, Sydney, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Centre for Virus Research, Westmead Millennium Institute, Sydney</wicri:regionArea><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author><author><name sortKey="Harman, Andrew N" sort="Harman, Andrew N" uniqKey="Harman A" first="Andrew N" last="Harman">Andrew N. Harman</name><affiliation wicri:level="3"><nlm:aff id="I1">Centre for Virus Research, Westmead Millennium Institute, Sydney, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Centre for Virus Research, Westmead Millennium Institute, Sydney</wicri:regionArea><placeName><settlement type="city">Sydney</settlement><region type="état">Nouvelle-Galles du Sud</region></placeName></affiliation></author></analytic><series><title level="j">Virology Journal</title><idno type="eISSN">1743-422X</idno><imprint><date when="2007">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The choice of an appropriate housekeeping gene for normalisation purposes has now become an essential requirement when designing QPCR experiments. This is of particular importance when using QPCR to measure viral and cellular gene transcription levels in the context of viral infections as viruses can significantly interfere with host cell pathways, the components of which traditional housekeeping genes often encode. In this study we have determined the reliability of 10 housekeeping genes in context of four heavily studied viral infections; human immunodeficiency virus type 1, herpes simplex virus type 1, cytomegalovirus and varicella zoster virus infections using a variety of cell types and virus strains. This provides researchers of these viruses with a shortlist of potential housekeeping genes to use as normalisers for QPCR experiments.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Australie</li></country><region><li>Nouvelle-Galles du Sud</li><li>Victoria (État)</li></region><settlement><li>Melbourne</li><li>Sydney</li></settlement><orgName><li>Université de Melbourne</li></orgName></list><tree><country name="Australie"><region name="Nouvelle-Galles du Sud"><name sortKey="Watson, Sarah" sort="Watson, Sarah" uniqKey="Watson S" first="Sarah" last="Watson">Sarah Watson</name></region><name sortKey="Bye, Chris" sort="Bye, Chris" uniqKey="Bye C" first="Chris" last="Bye">Chris Bye</name><name sortKey="Cunningham, Anthony L" sort="Cunningham, Anthony L" uniqKey="Cunningham A" first="Anthony L" last="Cunningham">Anthony L. Cunningham</name><name sortKey="Harman, Andrew N" sort="Harman, Andrew N" uniqKey="Harman A" first="Andrew N" last="Harman">Andrew N. Harman</name><name sortKey="Mercier, Sarah" sort="Mercier, Sarah" uniqKey="Mercier S" first="Sarah" last="Mercier">Sarah Mercier</name><name sortKey="Wilkinson, John" sort="Wilkinson, John" uniqKey="Wilkinson J" first="John" last="Wilkinson">John Wilkinson</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000E87 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000E87 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= CovidV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:2216015
|texte= Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:18053162" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a CovidV1
| This area was generated with Dilib version V0.6.33. |  |